Analysis Note

ControlARPE cell lysate

Application

Research Sub CategoryCytoskeleton

Anti-Vimentin Antibody, clone 3CB2 is an antibody against Vimentin for use in WB, IC, IH.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in human central and peripheral retinal sections (Perez-Alvarez, M.J., et al. (2008). J Neurosci Res. 86(8):1871-1883).

Western Blot Analysis:
A representative lot was used to detect vimentin in chicken and rat total brain extracts (Prada, FA., et al. (1995). GLIA 15:389-400).

Immunohistochemistry Analysis:
A representative lot was used to detect vimentin in E3 chick embryo sections and different regions of the developing chick central nervous system (Prada, FA., et al. (1995). GLIA 15:389-400).

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Vimentin is a class III intermediate filament protein (homopolymers) found in various non-epithelial cells including mesenchymal cells and fibroblasts. In many cells of mesenchymal origin, it is one of the most prominent phosphoproteins, especially during cell division when enhanced phosphorylation of vimentin corresponds with the reorganization of vimentin filaments.

Immunogen

Vimentin extracted from chicken embryonic brain tissue.

Physical form

Ion Exchange

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blot in ARPE19 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Vimentin in ARPE19 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~54 kDa observed